Table 1.

Drug loading in micelles was investigated by increasing RAP to polymer ratio (w/w) from 5 to 50%, while maintaining the concentration of the polymer at 0.5 mm and siRNA at 3 µm. RP-HPLC was used to quantify RAP encapsulation levels, and to calculate loading efficiency.

w/w RAP/ polymer	Initial RAP, mg/ml	RAP solubilized, mg/ml	RAP loaded, mg/mg	RAP: polymer, mol: mol	Efficiency, % loaded
5%	0.55	0.47 ± 0.02	0.041 ± 0.002	1.01 ± 0.05	85 ± 4
10%	1.10	0.69 ± 0.03	0.057 ± 0.002	0.43 ± 0.06	63 ± 3
25%	2.75	1.72 ± 0.10	0.125 ± 0.008	3.13 ± 0.19	63 ± 4
50%	5.50	3.19 ± 0.03	0.194 ± 0.002	4.84 ± 0.05	58 ± 1