TABLE 1.

Locomotor activity rhythms of CS, norpA^P41, and cry^b flies at different temperatures^a

Genotype	Temp (°C)	nb	Time (h) relative to ZT0 (mean ± SEM)d
			50% onsetc	Peak
CS	18	32	8.9 ± 0.2	10.4 ± 0.1
norpAP41	18	30	7.2 ± 0.3*	9.1 ± 0.2*
cryb	18	28	7.4 ± 0.2*	9.2 ± 0.2*
CS	29	31	10.6 ± 0.2	12.1 ± 0.2
norpAP41	29	30	8.5 ± 0.2*	11.6 ± 0.2**
cryb	29	27	9.3 ± 0.4*	11.5 ± 0.4

^aFlies were kept at either 18 or 29°C and exposed to 4 days of 12:12LD. Average data from two independent experiments are shown.

^bTotal number of flies that survived until the end of the testing period.

^cOnset is the time at which the upswing in evening activity reached 50% of the peak value and is given in hours from the last lights-on transition, where ZT0 is lights-on.

^d*, significant difference between CS and norpA or cry^b at P < 0.001 using Student's t test. **, significant difference between CS and norpA at P < 0.05. No significant differences were observed between norpA and cry^b for either onset or peak at either temperature.