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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Nature. 2013 Sep 11;501(7467):380–384. doi: 10.1038/nature12530

Figure 4. Usp16 contributes to proliferation defects and senescence in Ts65Dn fibroblasts.

Figure 4

a, Proliferation of TTFs seeded at P2. b, Senescent cells are frequent in Ts65Dn, but not in Ts65Dn/USP16het TTFs, as shown by SA-βgal and p16Ink4a staining at P3. Downregulation of cdkn2a blocks senescence. c, p16Ink4a and p19Arf mRNA levels rapidly increase during passaging in Ts65Dn MEFs. d, Downregulation of Usp16 normalizes mRNA expression of Ink4a/Arf by P6 Ts65Dn MEFs. e, ChIP analyses at the cdkn2a locus show lower levels of H2AK119 binding by two Ts65Dn MEF chromatin samples.