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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Nature. 2013 Sep 11;501(7467):380–384. doi: 10.1038/nature12530
Vendor Catalog # Item Description
Antibodies used for mouse hematopoiesis studies
eBioscience 15-0031-82 CD3e Anti-mCD3e PeCy5
Biolegend 100609 CD5 Antibody, anti-mouse CD5-PE/Cy5
eBioscience 15-0081-81 CD8a Antibody, anti-mouse CD8a-PE/Cy5
eBioscience 17-5931-81 Gr-1 Antibody, anti-mouse LY-6G (Gr-1)-APC
eBioscience 15-0452-82 B220 PeCy5 anti-mouse/human CD45R (B220)
Biolegend 116232 Ter119 Mouse Ter119-PacB
Biolegend 108120 Sca-1 Antibody, Pac Blue Sca-1 clone D7
eBioscience 47-1171-82 c-kit c-kit APC 780
Biolegend 115904 CD150 CD150 PE
Biolegend 103411 CD48 CD48 APC
eBioscience 17-1351-82 CD135 (Flt3) Anti-Mouse CD135 (Flt3) APC
eBioscience 11-0341-82 CD34 Anti-mouse CD34-FITC
eBioscience 12-0453-81 CD45.1 Antibody, anti-mouse CD45.1-PE
eBioscience 11-0454-81 CD45.2 Antibody, anti-mouse CD45.2-FITC
Biolegend 116232 Ter119 Mouse Ter119-PacB
Biolegend 101211 Mac-1 Antibody, anti-mCD11b(Mac-1)-APC
eBioscience 15-0031-82 CD3e Anti-mCD3e PeCy5
Antibody, anti-human/mouse
eBioscience 12-0452-81 B220 CD45R (B220)-PE
Antibodies used for murine mammary epithelial studies
Biolegend 313616 CD49f APC anti-human/mouse CD49f
Biolegend 101822 CD24 PECy7 anti-mouse CD24
Biolegend 103126 CD45 Mouse CD45-PacB
Biolegend 116232 Ter119 Mouse Ter119-PacB
Biolegend 102422 CD31 Mouse CD31-PacB
DSHB, University of Iowa TROMA-I CK8 TROMA-I, cytokeratin Endo-A,
marker for mouse endoderm
Covance PRB-155P-100 CK14 Keratin14(AF64)
Antibodies used for chromatin studies
Ubiquityl-histone H2A (Lys119)
Cell Signaling Tech 8240S (D27C4) XP rabbit mAb
Millipore 05-678 Antibody, ant ubiquityl histone h2a
Invitrogen A11001 Alexa Fluor 488 Alexa Fluor 488 goat anti-mouse IgG (H+L)
Invitrogen A11037 Alexa Fluor 594 Alexa Fluor 594 goat anti-rabbit IgG (H+L)
For Lineage Gating (Flow cytometry)
Biolegend 103133 CD45 Brilliant Violet 421™ anti-mouse
CD45 Antibody
Biolegend 102423 CD31 Brilliant Violet 421™ anti-mouse
CD31 Antibody
Biolegend 119321 CD34 Brilliant Violet 421™ anti-mouse
CD34 Antibody
Biolegend 116233 Ter119 Brilliant Violet 421™ anti-mouse
TER-119/Erythroid Cells Antibody
For Lineage Depletion (MACS)
Biolegend 103104 CD45 Biotin anti-mouse CD45 Antibody
Biolegend 102504 CD31 Biotin anti-mouse CD31 Antibody
Biolegend 119304 CD34 Biotin anti-mouse CD34 Antibody
Biolegend 116204 Ter119 Biotin anti-mouse TER-119/Erythroid
Cells Antibody
Miltenyi 130-090-485 anti-biotin Anti-Biotin MicroBeads
Antibodies for neural progenitor studies
BD Biosciences 340703 CD15 CD15 (SSEA-1, Lewis X)- FITC
eBioscience 17-1331-81 Prominin1 Anti-Mouse CD133 (Prominin-1) APC
Biolegend 101808 CD24 PE anti-mouse CD24 Antibody
Life Technologies E-3477 EGF Epidermal Growth Factor, Biotin-XX
Conjugate (biotin EGF)
Biolegend 405206 Streptavidin PE/Cy7 Streptavidin
Immunocytochemistry
Abcam ab97959 Sox2 Rabbit polyclonal Anti-SOX2 antibody
(ab97959)
Abcam ab6142 Nestin Anti-Nestin antibody [2Q178]
Fibroblast cell culture
cells
ATCC CCl-54 fibrobalsts Down Syndrome
ATCC CRL-7090 fibrobalsts Down Syndrome
ATCC CRL-7031 fibrobalsts Down Syndrome
ATCC CRL-2088 fibrobalsts Down Syndrome
ATCC CRL-2076 fibrobalsts Down Syndrome
Antibodies used for p16 detection
Santa Cruz sc-56330 p16 (JC8)
Invitrogen A11001 Alexa Fluor 488 Alexa Fluor 488 goat anti-mouse IgG
Invitrogen A21245 Alexa Fluor 647 Alexa Fluor 647 goat anti-rabbit IgG (H+L)