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Specificity of human RKIP and p-RKIP monoclonal antibodies. RKIP immunohistochemistry performed on formalin-fixed paraffin-embedded human brain tissue (A) and p-RKIP detected in human liver (B). Note the intense cytoplasmic and nuclear staining. Western blotting detection of 23kDa RKIP in 4 different cell lines (C) and p-RKIP (S153) in HeLa cells before – and after + treatment with calyculin A, the pp1 and pp2 phosphatases’ inhibitor (D). Courtesy of Epitomics.
