A.

Representative western blot analysis of ERα, e-cadherin, MTA3, Snail, RKIP and Tubulin in metastatic, triple negative (MDA-MB231) and non-metastatic (MCF7, T47D) breast cancer cell lines. Tubulin expression was served as a loading control. (B) Protein expression of RKIP and Snail in MCF7 cells expressing ERα specific or scramble control siRNA. (C) Representative western blot analysis of Snail and RKIP expression in protein lysates derived from MDA-MB231 cells expressing the ERα (C) or MTA3 (D) proteins. EVC: Empty vector control. Actin expression was used as a loading control. The experiments have repeated at least three times with very similar results. (E) A representative model depicting the mechanism through which RKIP expression is induced by ER.