Table 1. Western immunoblot evaluation of annexin 2 and caveolin expression in cultured human TM cells and microdissected porcine TM tissues.
| Protein | Function | Human TM Cells* |
Mircodissected perfused porcine TM* |
||||
|---|---|---|---|---|---|---|---|
| Control (PBS) | sCD44 treatedwith 0.1 ng for 24 h | Ratio | Control (DMEM) | sCD44 treated with 1 ng for 72 h | Ratio | ||
| Annexin 2 |
Junctional Assembly
Actin Cytoskeleton
Chloride Channel |
109 |
69.2 |
0.63 |
90.6 |
48 |
0.53 |
| Annexin 2 phosphorylated |
Negative modulator of actin assembly |
11 |
41.1 |
3.72 |
47.9 |
56.8 |
1.19 |
| Caveolin 1 |
Oligomers form lipid rafts |
51.4 |
60.2 |
1.17 |
19.9 |
86.2 |
4.34 |
| Caveolin 2 | Lipid rafts | 25.6 | 24.2 | 0.95 | 100 | 89.1 | 0.89 |
Protein loading control for each western blot was GAPDH and the protein abundance is expressed as densitometric units and normalized to Jurkat cell lysate as a positive control. *in 100,000s of densitometric units annexin 2 and caveolin 2 showed decreased concentrations in human and porcine TM cells when samples were treated with sCD44. Phosphorylated annexin 2 and caveolin 1 concentrations were increased in human and porcine TM cells when treated with sCD44. Comparison of densitometric data are an estimate of relative changes in protein concentration.