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. 2013 Nov 4;8(11):e79355. doi: 10.1371/journal.pone.0079355

Figure 3. Effect of FLAT upon AEA hydrolysis and uptake.

Figure 3

(A) AEA hydrolysis by HeLa cell homogenates transfected with FAAH or FLAT. Inset: Close up of AEA hydrolysis by FLAT-transfected and vector-transfected HeLa homogenates (n = 3). (B) Inhibition of FAAH and FLAT by URB597, PF-3845, and MAFP. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (n = 3-5). (C) AEA (100 nM) hydrolysis by FLAT normalized to its expression level relative to FAAH (n = 3). (D) FLAT hydrolyzes AEA (0.1-100 µM) with a Km of 25.3 ± 14.2 µM and Vmax of 0.29 ± 0.13 nmol/mg/min (n = 3-5). (E) AEA uptake in HeLa cells transfected with FAAH or FLAT in the absence or presence of URB597. *, p < 0.05 (n = 3). (F) Western blot showing equal expression of WT and S241A FLAT in HeLa cells. (G) Enzymatic activity of WT and S241A FLAT. (H) AEA uptake in HeLa cells expressing WT or S241A FLAT (n = 3).