Figure 3. Colocalization of 5HTR_A- and PTTH-ir in the early pupal BR-SOG of.

A. pernyi. BmPTTH-ir/ApPTTH-ir was co-localized with Ap5HTR_A-ir in the BR-SOG of 5-day-old pupa. (A) The location of detected cells. Lower-case letters correspond to the regions shown in the photographs (e.g., b to B). (B) Two large PTTH-ir neurons in the DL region. (C, D, H) Two large 5HTR_A-ir neurons in the DL region. (E) BmPTTH-ir in the DL region. (F) Merged image of BmPTTH- and 5HTR_A-ir in the DL region. (G) ApPTTH-ir in the DL region. (I) Merged image of ApPTTH-ir and 5HTR_A-ir in the DL region. Scale bar = 100 µm.