Table 1. A list of primers used in the experiments.
Name | Sequence of the primers |
---|---|
5HTRA-T7-F | TAATACGACTCACTATAGGGAGAAATACCTCCCGACTGTGTAAATATG |
5HTRA-T7-R | TAATACGACTCACTATAGGGAGACGTAATGTCACTTAAACAACAGGTG |
5HTRA-F | GATAGTTGACGGTAAAATCGTCGT |
5HTRA-R | AGCAGTTCCCGTCGTACCAG |
5HTRB-T7-F | TAATACGACTCACTATAGGGAGAATCAGAGGATCTAAGATGTGTCGTC |
5HTRB-T7-R | TAATACGACTCACTATAGGGAGACTAGATTGTTTTTCCGGGCTAGTAT |
5HTRB-F | TATGGCTAGGTTACTTCAACTCCAC |
5HTRB-R | GTTTCAAACTAGACGAGGTCAGTCA |
GFP-T7-F | TAATACGACTCACTATAGGGAGACCTGAAGTTCATCTGCACCAC |
GFP-T7-R | TAATACGACTCACTATAGGGAGAACGAACTCCAGCAGGACCAT |
RP49-F | AAGACCCGTCACATGCTACC |
RP49-R | GCGTTCGACGATTAACTTCC |
Underlined sequences are the T7 promoter and the highlighted sequences are the original primers.