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. Author manuscript; available in PMC: 2014 Sep 5.
Published in final edited form as: J Phys Chem B. 2013 Aug 23;117(35):10122–10131. doi: 10.1021/jp405397m

Figure 2.

Figure 2

HPLC-UV analysis of TpTpT irradiated with X-rays (10 kGy). TpTpT was irradiated at room temperature in the solid state with minimum hydration (2.5 mole H2O/mole nucleotide). Samples were dissolved in H2O and injected without further treatment (a), and after treatment with AP (b), to convert products with a terminal phosphate to a terminal hydroxyl group (black arrows). A control sample without radiation is shown in the bottom chromatogram (c).