Figure 8. Glucose transporter-1 (GLUT1) immunoreactivity increased in the capillaries of the lesion penumbra in rats on ketogenic diet.

(A) Representative pictures of spinal cord section showing GLUT1 staining characteristic of blood vessels at 7 days following injury (5x magnification; Leica DM5000B microscope, Leica Microsystems). Note the enhanced GLUT1 staining at the penumbra of the lesion cavity. To count number of Glut1 positive capillaries a 750×750 μm window was placed over the gray matter, with bottom of the window always touching boundary between gray and white matter (represented by box I). Scale bar: 500 μm (B) Higher magnification of representative field for GLUT1 quantification analysis of the SD and KD group, scale bar: 100 μm. (C) Blood vessel density, as measured by GLUT1 staining, increased by ~30% in the KD group compared to the SD group. Error bars indicate SEM. * p ≤ 0.05 (t-test).