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. 2013 Nov 4;8(11):e78876. doi: 10.1371/journal.pone.0078876

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© 2013 Wu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative images for western blot experiement on total (t-) and phosphorylated (p-) STAT-3. n = 9 for Ctrl group, n = 10 for HF group, and n = 11 for HP group, respectively. (B) Measurement of the p-STAT-3/total-STAT-3 ratio in saline injected rats and in leptin injected rats. For clearly comparison, all the p-STAT-3/t-STAT-3 ratios were normalized to the mean value that obtained from the Ctrl group. (C) The sensitivity toward leptin assessed by a significant elevation of the mean p-STAT-3/t-STAT-3 in leptin-injected compared to saline-injected rats. **p<0.001, compared as indicated.