Table 1. Strains and plasmids used in this study.

Strains	Characteristics	Source
B. subtilis NCBI-3610 (3610)	Undomesticated wild-type strain	Bacillus Genetic Stock Center (BGSC)
menG Mutant of B. subtilis 3610	menG::tn-spc, complex colony development formation defect	This report
yomI mutant of 3610	yomI::tn-kan	This report
lysA mutant of 3610	lysA::tn-kan, kanR,	This report
Mutant of 3610 carrying an insertion in a non-coding region upstream of a gene cluster consisting of yxaC, and yxaD	non-coding region upstream of a gene cluster consisting of yxaC, and yxaD::tn-kan	This report
dhbA mutant of 3610	dhbA::tn-kan, kanR,	This report
dhbE mutant of 3610	dhbE::tn-kan, kanR.	This report
dhbF mutant of 3610	dhbF::tn-kan, kanR	This report
Plasmids	Characteristics	Source
pIC333	A 2.4-kb mini-Tn10 transposon containing a ColEl origin of replication, a Spectinomycin resistance gene, a mls (Erythromycin + Lincomycin) resistance gene, a thermosensitive origin of replication for Gram-positive hosts (inactive at temperatures higher than 35°C)	[93]
pMarA	A TnYLB-1 transposon containing a Kanamycin resistance gene, an Erythromycin resistance gene, a thermosensitive origin of replication (repG+ts) for Gram-positive hosts (inactive at temperatures higher than 45°C).	[92]