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. 2013 Nov 4;8(11):e79841. doi: 10.1371/journal.pone.0079841

Figure 5. Enhanced Nrf2 activity decreases lipid clearance rate and VLDL secretion in Keap1-KD mice.

Figure 5

(A) Enhanced Nrf2 activity impairs lipid clearance. Ten-week-old C57BL/6 (WT) and Keap1-KD (KD) mice were fasted overnight (more than 16 hrs), and then administered olive oil (15ml/kg) by oral gavage, serum TG content was measured 0, 1, 3, and 6 hrs after oil administration (n=5 to 8 per group) (&, P<0.05, Keap1-KD compared with WT mice). (B) The expression of fatty acid transporters of CD36 and FATP2 was deceased in liver from Keap1-KD mice compared with WT mice by fasting (n=4 to 6 per group). VLDL secretion was impaired in Keap1-KD mice (C and D). Ten-week-old C57BL/6 (WT) and Keap1-KD (KD) fasted for 5 hrs were injected with Tyloxapol (0.5 mg/g) and serum TG content was measured at 0, 1, and 2 hrs after injection (n=5 to 8 per group) (&, P<0.05, Keap1-KD compared with WT mice). The induction of (E) ApoB and (F) MTTP expression was attenuated in liver of Keap1-KD mice by fasting. The relative mRNA levels were quantified by quantitative real-time PCR and normalized with 18S as loading control (n=4-6 per group). *, P<0.05, WT-Fasted compared with WT-Fed mice; #, P<0.05, KD-Fasted compared with KD-Fed mice; §, P<0.05, KD-Fasted compared with WT-Fasted mice.