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. 2013 Nov 4;8(11):e80109. doi: 10.1371/journal.pone.0080109

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© 2013 Kim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Prep-HPLC chromatography was performed to detect rhG-CSF protein. A. Chromatogram of prep-HPLC with the sup after pH precipitation of refolded rhG-CSF. B. IEF analysis of each fraction from A to H obtained by Prep-HPLC. The IEF marker indicates pI. Lane 1, pI marker 4.5–7.4. Absorbance is in milliabsorbance units (mAU).