Table 1. Summary of each step during the purification of rhG-CSF.

Process Steps	Total Mass (gram/Mean±S.D.*)	Yield	Purity (%)	Remark Weight
Cell Paste	134±6.7	-	-	Amount of Water content: 80%
Washed IB	47.5±1.9	-	-	Amount of Water content: 65%
Refolding	3.87±0.19	65	70	RP-HPLC & SDS-PAGE
Acidic Precipitation	2.69±0.094	69	85	RP-HPLC & SDS-PAGE
Ultrafiltration	2.31±0.035	86	87.5	RP-HPLC
Prep-HPLC	1.75±0.079	76	99	RP-HPLC

^*Each value represents the mean of five performances ± S.D.