Figure 3. Co-culture with HO-1-induced PC3 cells decreased the expression of osteoclast-modulating factors in osteoblasts.

Experiments were done in PMOs grown alone (PMO), co-cultured with PC3 (PMO PC3) or with PC3 pre-treated with hemin (PMO PC3 Hem). Total RNA was extracted and RANKL (A), OPG (B), CSF-1 (C), OPN (D), CCL2 (E) and IL-6 (F) mRNA levels were analyzed by RT-qPCR. Data were normalized to β-actin and were expressed as fold induction respect to PMOs. One representative from at least three independent experiments is shown (Significant difference, * P<0.05; ** P<0.01; *** P<0.001).