Figure 3. Human AIM enhances foam cell formation and IL-8 secretion.

A) THP1 MФ were infected with M. tuberculosis at MOI 0.1 for 24 h in RPMI 1% FBS medium, fixed, stained with Nile Red and observed by fluorescent microscopy (left panel), or quantified by flow cytometry (right graphic). MFI, Median Fluorescence Intensity. B) The amount of IL-8 in culture supernatants from M. tuberculosis-infected THP1 MФ at the indicated MOIs during 24 h was determined by ELISA. Mean ± SEM from three independent experiments performed in triplicate are shown. *p≤0.05; **p≤0.01; two-way ANOVA.