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. 2013 Jun 13;10(8):1312–1323. doi: 10.4161/rna.25274

graphic file with name rna-10-1312-g1.jpg

Figure 1. HuR protects pancreatic cancer cells against glucose-deprivation. In bar graphs: siCTRL, black bars; siHuR, gray bars (*p < 0.05). In survival curves (unless indicated): siCTRL are solid lines; siHuR are dashed lines. (A) Representative qPCR 24 h after transfection in MiaPaCa2 cells. (B) Representative immunoblot 48 h after transfection in MiaPaCa2 cells. (C) Annexin V staining in MiaPaCa2 cells. (D) Trypan blue staining in MiaPaCa2 cells. (E) PicoGreen assays at 7 d in MiaPaCa2 cells. (F) Three-week soft-agar colony formation assays in MiaPaCa2 cells. PicoGreen assays at seven days in (G) BXPC3 and (G) PANC1 cells. (I) Representative immunoblot 72 h after transfection in PANC1 cells. (J) PicoGreen assay at seven days in PANC1.HuR (double line-top) vs. PANC1.EV (single line-bottom).