Figure 2. HuR protein is activated to the cytoplasm under glucose deprivation. (A) Immunoblots of whole cell (left) and cytoplasmic (right) lysates from MiaPaCa2 cells cultured for 6 h in media with the indicated glucose concentrations. Gemcitabine (GEM) (1 µM) was used as a positive control for cytoplasmic HuR activation. HuR (and GEM) levels are quantified at each glucose concentration and numeric values are displayed normalized to control glucose conditions below the corresponding cytoplasmic HuR band. Immunofluorescence at 12 h (B) and immunoblots at 6 h (C) of BxPC3 cells in normal DMEM (25 mM glucose), 1 mM glucose DMEM and 1 µM gemcitabine (positive control). Nuclei are counterstained in blue (DAPI). Immunoblots of (D) PANC1 cells after incubation in the indicated glucose concentrations for 6 h. Relative HuR levels are quantified, as described for panel (A).