Figure 4. Novel small RNAs derived from intergenic regions and introns are selectively packaged in glioma microvesicles. (A) Bar graph showing the abundance of the top 20 intergenic loci producing small RNAs found almost exclusively in microvesicles. Inset histogram shows the characteristic stacked distribution of reads at a representative locus. (B) Bar graph showing the abundance of small RNAs mapping to introns in a sense direction to the gene shown on the y-axis. Inset histogram shows the characteristic stacked distribution of reads at representative locus. (C) Same as in (B) except the small RNAs map in an antisense direction to the genes indicated on the x-axis. Note that these antisense intronic small RNAs are found almost exclusively in the microvesicles and not the parent U251 cells.