Figure 1. Localization of the chr promoter within the tnpR-chrB intergenetic region using gfp transcriptional fusions.

A) PCR fragments containing the promoter portions (indicated by the boxes) were cloned upstream of a promoterless gfp on pProbe-NT. Sequences are numbered relative to the first nucleotide of the chrB start codon. Primer pairs used in this work are indicated. B) Green fluorescence of E. coli cells harboring the different constructs. The values represent averages and standard deviations of three replicates.