Figure 3. Study of the functionality of ChrB (total or partial) using reporter plasmids.

A) Total or partial chrB (coding for N-terminal or C-terminal ChrB), with their promoter sequence, were cloned upstream of gfp gene in pProbe-NT (Table1). Primer pairs used in this work are indicated. B) Fluorescence of E. coli carrying the different plasmids constructs; pChrBGFPn (white bars), pChrBGFPc (grey bars) and pChrBGFP (dashed bars). Fluorescence was measured after 3 h of growth in medium without chromate and with increasing chromate concentrations. The values represent averages and standard deviations of three replicates.