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. 2013 Nov 4;8(11):e77987. doi: 10.1371/journal.pone.0077987

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© 2013 Branco, Morais

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) SDS PAGE of purified ChrB in glutaraldehyde crosslinking assays: lane 1, ChrB; lane 2, ChrB+glutaraldehyde (2 min); lane 3, ChrB+glutaraldehyde (5 min); lane 4, ChrB+glutaraldehyde (10 min); lane 5, ChrB+glutaraldehyde (15 min); lane 6, ChrB+glutaraldehyde (30 min); lane 7, ChrB+glutaraldehyde (1 hour). B) Immunoblot of purified ChrB-His6 in glutaraldehyde crosslinking assays: lane 1, ChrB-His6; lane 2, ChrB-His6+ glutaraldehyde; lane 3, ChrB-His6+ target chr promoter DNA+glutaraldehyde; lane 4, ChrB-His6+ glutaraldehyde+Cr(VI).