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. 2013 Nov 4;8(11):e77987. doi: 10.1371/journal.pone.0077987

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© 2013 Branco, Morais

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Green fluorescence of pChrBGFP (ChrB no mutated) and pChrBGFP mutants, in which, ChrB is mutated in R175A; R180A; R182A; R187A; R195A; R196A; C213A, H229A and R258A. B) Green fluorescence of pChrBGFP mutants, in which, ChrB is mutated in R18A; R23A; A241R; G244R and R252A. Fluorescence was measured after 3 h of growth in medium without chromate (white bars), with 1 µM (black bars) and 10 µM (dashed bars) of chromate. The values represent averages and standard deviations of three replicates.