Instrument |
X33 (EMBL, DORIS ring, DESY) |
Beam geometry |
|
2 mm×0.6 mm |
Wavelength [Å] |
|
1.5 |
range [Å−1] |
|
0.0074-0.5 |
Exposure time [s] |
|
8×15 |
Temperature [K] |
|
283 |
Concentration range [mg/ml] |
|
2.2–3.8 |
|
|
0.7–1.2 |
Structural parameters
|
|
|
|
[% ] from P(r) |
|
53±5 |
32.25±12 |
|
|
[Å ] from P(r) |
|
31.2±1.2 |
18.8±0.1 |
[% ] from Guinier |
|
72.8±9 |
31.6±0.06 |
|
|
[Å ] from Guinier |
|
29.3±2 |
17.8±2.1 |
[Å] from data |
|
117 |
61.4 |
|
|
[Å ] |
Model 1
|
30 |
17.4 |
|
|
[Å ] |
Model 2
|
33 |
|
[Å] of model envelope |
Model 1
|
112 |
61.43 |
|
Model 2
|
124 |
|
Porod volume estimate [Å] |
|
30230 |
10950 |
Dry volume calculated from model [Å] |
Both models
|
28900 |
14100 |
fit |
Model 1 |
0.75 |
1.07 |
|
Model 2 |
0.93 |
|
Molecular-mass determination
|
|
|
Molecular mass from I(0) in P(r) [kDa] |
|
18±2 |
10.7±3 |
Calculated monomeric from sequence [kDa] |
|
23.4 |
10.3 |
Calculated monomeric from I(0) in Guinier [kDa] |
|
25±3 |
11.6 |
Software employed
|
|
|
Primary data reduction |
|
PRIMUS |
Data processing |
|
GNOM |
Ab initio analysis |
|
DAMMIF |
Model comparison |
|
SUPCOMB |
Tertiary structure modelling |
|
RFR (this paper)
|
Computation of model intensities |
|
CRYSOL |
Three-dimensional graphics representations |
|
PyMol |