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. 2013 Nov 4;8(11):e78033. doi: 10.1371/journal.pone.0078033

Figure 6. FLZ decreased the expression of BACE1 and did not affect the expression of IDE.

Figure 6

APP/PS1 double transgenic mice were orally treated with FLZ 150 mg/kg for 20 weeks. SH-SY5Y (APPwt/swe) cells were grown in “stimulating medium” containing 50% DMEM, 50% Opti-MEM, 0.5% FBS, 200 µg/ml G418 and 10 mM butyric acid sodium salt for 12 h to induce the transgene expression. FLZ (0.1, 1 and 10 µM) were incubated with cells for 24 h. (A) Western blot assay of BACE1 and IDE in the hippocampus of APP/PS1 mice treated with FLZ. A representative immunoblot from 4 mice was shown. Results are expressed as mean±SD. *P<0.05 vs. WT mice; # P<0.05 vs. APP/PS1 mice. (B) Western blot assay of BACE1 and IDE in SH-SY5Y (APPwt/swe) cells treated with FLZ. A representative immunoblot from four independent experiments was shown. Results were expressed as mean ± SD. **P<0.01 vs. Neo SH-SY5Y cells, # P<0.05, ## P<0.01 vs. solvent-treated SH-SY5Y (APPwt/swe) cells.