Figure 7. FLZ attenuated tau phosphorylation.

APP/PS1 double transgenic mice were orally treated with FLZ 150 mg/kg for 20 weeks. SH-SY5Y (APPwt/swe) cells were grown in “stimulating medium” containing 50% DMEM, 50% Opti-MEM, 0.5% FBS, 200 µg/ml G418 and 10 mM butyric acid sodium salt for 12 h to induce the transgene expression. FLZ (0.1, 1 and 10 µM) were incubated with cells for 24 h. Antibodies against p-tau (Ser396), p-tau (Ser202/Thr205), p-tau (Thr231) and p-tau (Ser404) were subjected to Western blot assay. A representative immunoblot from 4 mice was shown. Results were expressed as mean±SD. (A) Western blot result in hippocampus of APP/PS1 mice. *P<0.05, **P<0.01 vs. WT mice; ^# P<0.05,^## P<0.01 vs. APP/PS1 mice. (B) Western blot result of SH-SY5Y (APPwt/swe) cells. **P<0.01 vs. Neo SH-SY5Y cells, ^# P<0.05, ^## P<0.01 vs. solvent-treated SH-SY5Y (APPwt/swe) cells.