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. 2013 Nov 4;8(11):e78083. doi: 10.1371/journal.pone.0078083

Figure 3. NgBR regulates estradiol-induced survivin expression in estrogen receptor positive breast tumor cells.

Figure 3

T47D is an estrogen receptor positive breast tumor cell line. MDA-MB-468 is an estrogen receptor negative breast tumor cell line. NgBR was knockdown in both T47D and MDA-MB-468 cells using siRNA as described in methods. Both tumor cells were treated with 10 nM estradiol for 48 hours. Protein levels of NgBR, ER-alpha and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. (A) NgBR knockdown diminished estradiol-induced survivin expression in T47D breast tumor cells. (B) Quantitative analysis of survivin protein level change in T47D cells by measuring intensity of survivin western blot band. Data is presented as fold changes of estradiol treatment group as compared to the non-treatment group (n = 3; * siNgBR vs NS p<0.05). (C) NgBR knockdown has no effect on survivin expression in MDA-MB-468 breast tumor cells. (D) Quantitative analysis of survivin protein level change in MDA-MB-468 cells by measuring intensity of survivin western blot band. Data is presented as fold changes of estradiol treatment group as compared to the non-treatment group. (n = 3; siNgBR vs NS p = 0.092).