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. 2013 Nov 4;8(11):e78083. doi: 10.1371/journal.pone.0078083

Figure 4. NgBR is essential for estradiol-induced survivin expression and cell growth of MCF-7 breast tumor cells.

Figure 4

MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. (B) Folds of survivin increase were determined by measuring relative western blot intensity of survivin. Data is presented as fold changes of estradiol treatment group as compared to the non-treatment group (n = 3; 6 hrs estradiol treatment, NS vs siNgBR p = 0.178; * 48 hrs estradiol treatment, NS vs siNgBR p<0.05). (C) NgBR knockdown impaired estradiol-stimulated cell growth of MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 24 and 48 hours. Viable cell numbers were counted using the Bio-Rad TC10™ Automated Cell Counter. Data is presented as mean±SEM (n = 3, # 24 hrs or 48 hrs estradiol treatment vs baseline p<0.05; * siNgBR vs NS p<0.05); E2: estradiol.