Figure 5. Stability of exosomal miRNAs under in vitro culture conditions.

Exosomes, isolated using differential ultracentrifugation from follicular fluid, were incubated under in vitro cell culture conditions (37°C & 5% CO₂) for 6 hr, 12 hr and 24 hr in a exosome-free culture medium in order to determine the stability of exosomal miRNAs by quantitative real time PCR. Non-cultured exosomes (0 hr) were used as reference controls to check the stability of exosomes coupled miRNAs in different time point. The data is presented as means and SD of three biological replicates.