Figure 5. E2 inhibits K⁺ recycling in normal and CF bronchial cell lines.

A. NuLi-1 (grey bars) and CuFi-1 (white bars) epithelial monolayers were stained with Calcein Green and ASL was stained with dextran conjugated to Texas Red^TM fluorochrome and ASL height was measured after treatment with E2 (30 min, 1 nM), and a cocktail of BaCl₂ and TEA to inhibit potassium channels (B/T 30 min, BaCl₂ 10 mM, TEA 1 mM) or pretreated with E2 and then treated with the K⁺ channels cocktail inhibitor (E2 + B/T) (n = 5, Error bars reflect standard error of the mean, ANOVA, * p<0.05, ** p<0.01). B. NuLi-1 (grey bars) and CuFi-1 (white bars) epithelial monolayers were treated with E2 (30 min, 1 nM), and the KCNQ1 channel specific inhibitor HMR1556 (HMR, 30 min, 1 µM) or pretreated with E2 and then treated with HMR1556 (E2 + HMR) (n≥3, Error bars reflect standard error of the mean, ANOVA, ** p<0.01).