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. 2013 Nov 4;8(11):e78235. doi: 10.1371/journal.pone.0078235

Figure 3. Detection of protein N-myristoylation in in vitro-synthesized protein Lunapark by MS analysis.

Figure 3

A. Purified in vitro-synthesized protein Lunapark (1 µg) was separated by electrophoresis in an SDS-PAGE gel (12.5%). B. MALDI-MS of the tryptic peptides from in vitro-synthesized protein Lunapark. The peaks of the tryptic peptides derived from in vitro-synthesized protein Lunapark are indicated by stars. C. MS/MS analysis was performed for the peak at m/z 846.55. The identified N-myristoylated N-terminal sequence is shown.