Figure 5. Protein N-myristoylation plays a critical role in the ER morphological change induced by protein Lunapark.
A. Detection of protein N-myristoylation of Lunapark-FLAG expressed in HEK293T cells. cDNAs encoding Lunapark-FLAG and Lunapark-G2A-FLAG were transfected in to HEK293T cells, and their expression and the N-myristoylation of the products in total cell lysates were evaluated by Western blotting analysis and [3H]myristic acid ([3H]Myr) labeling, respectively. B. Intracellular localization of Lunapark-FLAG and Lunapark-G2A-FLAG was determined by immunofluorescence staining of HEK293T cells transfected with cDNAs encoding these two proteins using an anti-FLAG antibody. a and b show a close-up view of the immunofluorescence image.