Figure 1. Degradation of poly(P) by Sf9 cell culture supernatant containing rh-TRAP.

The [³²P]-poly(P)₄₀ (panel A) or [³²P]-poly(P) with an average chain length of 40, 300, or 750 residues (panel B) (0.346 mM) were incubated with Sf9 cell culture supernatant containing rh-TRAP (7.3 mU/mL) in 100 mM Na-acetate buffer (pH 5.5) with 40 mM sodium tartrate for the indicated time periods at 37°C. Degradation products were analyzed by 20% PAGE.