Table 1. Polyphosphatase activity as well as phosphatase activity of rh-TRAP after co-precipitation with anti-TRAP antibodies.

	Buffer	Before co-precipitation	Sup. (15A4)	Pellet (15A4)	Sup. (13B9)	Pellet (13B9)
Polyphosphatase	23.8±12.7	100.0*	22.7±18.3	103.3±6.08**	20.4±24.1	101.1±37.6***
Phosphatase	11.5±1.03	100.0#	21.7±0.80	88.7±2.95##	16.4±0.50	95.7±2.25###

The rh-TRAP was separated from Sf9 cell culture supernatant by co-precipitation with anti-TRAP antibodies (15A4 and 13B9). Polyphosphatase activity was also measured using [³²P]poly(P)₄₀ (0.346 mM) as substrate. Phosphatase activity was measured using pNPP as substrate. These activities were evaluated using the supernatant fraction (Sup.) or the pellet fractions (Pellet) after co-precipitation with anti-TRAP antibodies, 15A4 or 13B9. Values are expressed as means±SD of the percentage from three independent experiments comparing with the activities of Sf9 cell culture supernatant before co-precipitation as 100%. *p<0.05, significantly different from polyphosphatase activity of buffer. **p<0.05, significantly different from polyphosphatase activity of Sup. (15A4). ***p<0.05, significantly different from polyphosphatase activity of Sup. (15B9). ^# p<0.001, significantly different from phosphatase activity of buffer. ^## p<0.001, significantly different from phosphatase activity of Sup. (15A4). ^### p<0.001, significantly different from phosphatase activity of Sup. (13B9). (Student's t-test).