Figure 3.

Effect of the selective Dectin-1 agonist, depleted zymosan, on B cell proliferation and antibody production. Isolated total spleen cells and purified spleen B cells from C57BL/6 were stimulated with depleted zymosan (dzn, 100 µg/ml), LPS (1 µg/ml) and anti-IgM (10 µg/ml). (A) After 2 days of culture, cell proliferation was measured by EZ-Cytox cell viability assay. Data are averages of duplicate samples with ranges (bars). (B) After 7 days of culture, supernatants were harvested, and levels of Igs production were determined by isotype-specific ELISA. Data represent means±SEM of triplicate samples. ^*p<0.05.