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. 2013 Oct 1;32(21):2861–2871. doi: 10.1038/emboj.2013.217

Figure 3.

Figure 3

Deletion of HPR1 or THP2 increases DNA:RNA hybrid (R-loops) formation at telomeres. (A) Deletion of HPR1 increases R-loop formation at telomeres. Three independent colonies of wt and hpr1Δ mutants were used to immunoprecipitate R-loops using the S9.6 antibody. Treating one half of each sample with RNase H and using an unspecific IgG antibody served as controls for the specificity of the antibody. The immunoprecipitated telomeres 1L, 7L, 10R, 15L, 3*Y′, 4*Y′ and 6*Y′ were amplified by qPCR and expressed as a percentage of input. Amplification of PMA1 3′ served as a positive control for R-loop formation as in Figure 1. Values of three independent biological replicates with standard deviation are shown. PMA1 controls and statistical analyses are as in Figure 1. (B) Deletion of THP2 increases R-loop formation at telomeres. Three independent colonies of a wt strain and of a thp2-Δ mutant were analysed by DIP as described in (A). Statistical analyses were calculated using the Student’s t-test (*P<0.05, **P<0.03 and ***P<0.01).