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. Author manuscript; available in PMC: 2013 Nov 5.
Published in final edited form as: Methods Mol Biol. 2013;1077:10.1007/978-1-62703-637-5_16. doi: 10.1007/978-1-62703-637-5_16

Fig. 3.

Fig. 3

Glutamine oxidation in uninfected, SCR, and SIRT4 KD Huh7 cells. Seahorse assay was run with the assay media containing 4 mM Glutamine. Data represents the average of five replicates for each cell line and has been normalized to the total protein amount. Basal OCR were at the maximal level, while basal ECRA were at minimal level in this media condition for all three analyzed cell lines. The glutamine oxidation in SIRT4 KD cells was the highest comparing to SCR and uninfected Huh7 cells. Again, the OCR difference was more significant when comparing to SCR (p = 0.0089) than comparing to the uninfected Huh7 cells (p = 0.32)