Skip to main content
. Author manuscript; available in PMC: 2013 Nov 5.
Published in final edited form as: Methods Mol Biol. 2013;1077:10.1007/978-1-62703-637-5_16. doi: 10.1007/978-1-62703-637-5_16

Fig. 4.

Fig. 4

Glucose oxidation in uninfected, SCR, and SIRT4 KD Huh7 cells. Seahorse assay was run with the assay media containing only 25 mM glucose. Data represents the average of six or seven replicates for each cell line and has been normalized to the total protein amount. With the presence of glucose in assay media, ECAR remained at regular level and no significant difference was observed in the three analyzed cell lines. Basal OCR was again increased in SIRT4 KD cells. The increase in basal OCR in SIRT4 KD cells was more significant when comparing to the SCR control (p = 0.00037) than to the uninfected Huh7 cells (p = 0.08)