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. 2013 Aug 27;23(11):1270–1283. doi: 10.1038/cr.2013.116

Figure 2.

Figure 2

α7nAChR activation upregulates miR-124. (A) q-PCR of miRNAs in RAW264.7 cells stimulated with 100 ng/ml LPS in the absence or presence of 5 mM nicotine for 9 h. (B) q-PCR of miR-124 in mice peritoneal macrophages stimulated with 1 ng/ml LPS in the absence or presence of 0.1 nM nicotine for 9 h. (C) Time courses of miR-124 induction in RAW264.7 cells stimulated with 100 ng/ml LPS with or without 5 mM nicotine. IL-6 and TNF-α productions in the supernatant were determined by ELISA. (D) q-PCR of miR-124 in peritoneal macrophages of α7nAChR+/+ or α7nAChR−/− mice treated with 1 ng/ml LPS with or without 0.1 nM nicotine for 9 h. (E) q-PCR of miR-124 in RAW264.7 cells pre-incubated with vehicle, 5 mM nicotine, 5 mM nicotine in combination with 10 μM MLA and then stimulated with 100 ng/ml LPS for 9 h. (F) q-PCR of miR-124 in RAW264.7 cells pre-incubated with 5 μM PNU for 10 min and then stimulated with vehicle or 100 ng/ml LPS for 9 h. Data represent mean ± SD (n = 3). NS, not significant (P > 0.05); **P < 0.01 by Student's t-test.