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. 2013 Aug 27;23(11):1270–1283. doi: 10.1038/cr.2013.116

Figure 5.

Figure 5

miR-124 targets STAT3 to inhibit IL-6 production. (A) Luciferase activity of HEK293 cells 24 h after transfection with the reporter containing 3′-UTR of STAT3, IL-6 or TNF-α mRNA and miR-124 mimic. (B) Western blotting of STAT3 protein in RAW264.7 cells transfected with miR-124 mimic or inhibitor and their corresponding controls for 36 h. (C) Western blotting of STAT3 and Y705-phosphorylated STAT3 in RAW264.7 cells transfected with the miR-124 mimic or negative control for 36 h, and then stimulated with vehicle or 100 ng/ml LPS for 9 h. (D) ELISA and q-PCR detection of IL-6 or TNF-α in RAW264.7 cells transfected with STAT3 siRNA or control siRNA for 48 h and then treated with 100 ng/ml LPS for 9 h. (E) ELISA detection of IL-6 or TNF-α in the supernatants of RAW264.7 cells transfected with STAT3 siRNA and miR-124 mimic for 36 h and then treated with 100 ng/ml LPS for 9 h. (F) ELISA and q-PCR detection of IL-6 in STAT3+/+ or STAT3−/− MEFs stimulated with 1 μg/ml LPS for 9 h. For B and C, data are representative of three independent experiments. For A and D-F, data represent mean ± SD (n = 3). NS, not significant (P > 0.05); **P < 0.01 by Student's t-test.