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Detection and phenotype of the progeny of single antigen-specific CD8+ T cells in response to viral infection. OVA-specific CD8+ T cells from the spleen were identified by OVAKb tetramer staining, and donor populations were identified by CD45.1 and CD45.2 staining within the OVA-Kb+ population. Expression of KLRG1 and CD127 was then analyzed on gated donor populations. This experiment was repeated 3 times, with 60 mice total.
