Table 1.
TCRα and TCRβ sequencing to prove clonality.
| TCRα sequences | |||
|---|---|---|---|
| Alpha-V | Alpha-J | CDR3 | |
| 1-OVA | TRAV 5-4 | TRAJ49 | AATSNTGYQNY |
| 2-OVA | TRAV6-1 | TRAJ12 | VLGGTGGYKVV |
| 3-OVA | TRAV14-2 | TRAJ22 | AASRPSGSWQLI |
| 4-N | TRAV7-3 | TRAJ15 | AVTPQGGRALI |
| 5-N | TRAV9 | TRAJ40 | VLSPNTGNYKYV |
| 6-OVA | TRAV4 | TRAJ12 | AADTGGYKVV |
| 7-N | TRAV7-4 | TRAJ26 | AASEEANYAQGLT |
| 8-N | TRAV6-5 | TRAJ22 | ALGASSGSWQLI |
| 9-N | TRAV6-3 | TRAJ27 | AMSDLPNTGKLT |
| TCRβ sequences | ||||
|---|---|---|---|---|
| Beta-V | Beta-J | Beta-D | CDR3 | |
| 1-OVA | TRBV14 | TRBJ1-5 | TRBD1 | ASSLGWNNNQAPL |
| 2-OVA | TRBV31 | TRBJ2-2 | TRBD2 | AWGLGLANTGQLY |
| 3-N | TRBV14 | TRBJ1-2 | TRBD1 | ASSFGANSDYT |
| 4-N | TRBV13-1 | TRBJ1-2 | TRBD1 | ASSQGRSSDYT |
| 5-N | TRBV29 | TRBJ1-1 | TRBD1 | XSSLSTAPLEVF |
Progeny of single CD8 T cells following VSV-OVA infection were sorted and the expressed TCR V-regions were sequenced. TCRα sequences for 9 clones and TCRβ sequences of 5 clones are shown. OVA or N specificities are indicated. See also Figure S1.