Figure 7.

Metformin potentiates the effect of carboplatin in 3three of the 8 primary cultures of ovarian cancer. A, Ovarian cancer cells isolated from peritoneal fluid (ascites) were cultured and incubated for 24 hours with either vehicle or metformin 20 µmol/L, followed by either vehicle or metformin for a further 48 hours, with stated increasing concentrations of chemotherapy. Each graph represents an individual patient. Solely due to limitations in cancer cell number, paclitaxel or carboplatin, were evaluated in patients 1 to 3 (Figure 5A), while patients 4 to 8 were also evaluated for response to doxorubicin (Figure 5B). The treatments under comparison correspond to “Veh 24-Veh 48” and “Met 24-Met 48” used in Figure 1. All data points have 5 replicates. #Significant variance from the replicates in an individual patient.