Figure 5.

Effect of hyperglycemia upon ¹⁴C-l-methionine (¹⁴C-l-Met) uptake by Bewo cells. Cells were exposed to 10 or 30 mmol/L d-glucose (n = 6-13) or mannitol (control; corresponding to 100%) for 1 to 72 hours, and initial rates of uptake were then determined by incubating cells for 6 minutes at 37°C in buffer with 250 nmol/L ¹⁴C-l-Met. Shown are arithmetic mean ± standard error of the mean (n = 6-13). *Significantly different from control (P < .05).