Figure 6.

Effect of insulin upon ¹⁴C-l-methionine (¹⁴C-l-Met) uptake by Bewo cells. (A) Cells were exposed to 0.01, 1, or 50 nmol/L insulin or the respective solvent (control; corresponding to 100%) for 1 to 48 hours, and initial rates of uptake were then determined by incubating cells for 6 minutes at 37°C in buffer with 250 nmol/L ¹⁴C-l-Met (n = 9-14); (B) cells were exposed to 0.01, 1, or 50 nmol/L insulin or the respective solvent (control, corresponding to 100%) for 1 or 4 hours, and initial rates of uptake were then determined by incubating cells for 6 minutes at 37°C in fetal calf serum (FCS)-free culture medium with 250 nmol/L ¹⁴C-l-Met (n = 8-13). Shown is arithmetic mean ± standard error of the mean. *Significantly different from control (P < .05) and ^#significantly different from insulin 0.01 nmol/L (P < .05).