Table 2.

Primer Sequences and ATs Used for Real-Time RT-PCR.

Gene Name	Primer Sequence (5′-3′)	AT, °C
β-Actin	Fwd: AGA GCC TCG CCT TTG CCG AT	65
	Rev: CCA TCA CGC CCT GGT GCC T
LAT2	Fwd: TCG CTG TGA CTT TTG GAG A	64
	Rev: GCC GAG AGG TGA AGA GA
SNAT1	Fwd: ACT ACC CTC TGC CAT AAA	60
	Rev: TAT AGC CAA GAT ACC CTA AGT
SNAT2	Fwd: GTC ATT GGT GGT CAT TCT T	60
	Rev: GTG GTG TTT ATT GTT TCG TTA
y+LAT1	Fwd: AAC TGT GCC AGG GAC ACT	65
	Rev: GAG AAG AGG GCA GAG TAG AGG

Abbreviations: LAT2, l-type amino acid transporter 2; SNAT1, sodium-coupled neutral amino acid transporter 1; SNAT2, sodium-coupled neutral amino acid transporter 2; y⁺LAT1, y⁺ l-amino acid transporter 1; Fwd, forward; Rev, reverse; RT-PCR, reverse transcription-polymerase chain reaction; AT, annealing temperature.