Table 1.
Gene | Primer sequences | AT | C | Size (bp) |
---|---|---|---|---|
p53 | Forward—CTG CCC ACC ACA GCG ACA GG | 59°C | 35 | 471 |
Reverse—AGG AGC CAG GCC GTC ACC AT | ||||
Bcl-2 | Forward—GGG CTA CGA GTC GGA TAC | 53°C | 35 | 64 |
Reverse—AGG CTG GAA GGA GAA GAT G | ||||
Bax | Forward—CGC GTG GTT GCC CTC TTC TAC TTT | 59°C | 35 | 124 |
Reverse—CAA GCA GCC GCT CAC GGA GGA | ||||
Caspase 9 | Forward—TGC AGG GTA CGC CTT GTG CG | 61°C | 35 | 130 |
Reverse—CCT GAT CCC GCC GAG ACC CA | ||||
Caspase 3 | Forward—AGG CCT GCC GAG GTA CAG AGC | 61°C | 35 | 255 |
Reverse—CCG TGG CCA CCT TCC GCT TA | ||||
18s | Forward—AAG ACG AAC CAG AGC GAA AG | 56°C | 25 | 149 |
Reverse—GGC GGG TCA TGG GAA TAA |
AT: annealing temperature; C: number of cycles during exponential phase of amplification.